Detection of Cpy-2, Fks and Erg 11 Genes in Fungal Isolates from Sars Cov-2 Individual in Ibadan, Oyo State, Nigeria

A considerable number of fungal strains have developed resistant to various available antifungal agents due CPY, FKS and or ERG11 genes complicating coinfection cases SAR COV-2 virus. Therefore, this study sought isolate, identify azole polyene in pathogens isolated from confirmed SARS-CoV-2 individual Oyo State, Nigeria. Nasopharyngeal samples were collected symptomatic asymptomatic infected adult September, 2020 April, 2021. Samples cultured on Sabouraud Dextrose Agar at room 37 oC temperature for 7days. Identification the isolates performed using MALDITOF MS VITEK. Antifungal Susceptibility Testing (AFST) Kirby bauer disc diffusion method. The fugal determined by Polymerase Chain Reaction with specific primers amplified agarose gel electrophoresis. Out 63(15.8%) recorded 400 collected, Asipergillus flavus 11(17.5%), Aspergillus niger 9(14.3%), Candida albicans 7(11.1%), guillermondii 2(3.2%), parapsilosis famata tropicalis 5(7.9%) Lodderomyces elongisporus 25(39%) having highest frequency respectively. Nystatin (84.1%) had susceptibility testing Ketoconazole (39.7%) least phenotypically. 10 (52.6%) possessed CPY gene, 8(42.1%) carried 9(47.4%) gene molecularly.